![]() ![]() ![]() ![]() This approach increases the yield of cNDs by over 10-fold and is able to rapidly generates cNDs with diameters ranging from 11 to over 100 nm. We herein introduce a simple, one-step approach to ease the construction of cNDs using the Sp圜atcher-SpyTag technology. However, previous methods for building cNDs require multiple steps and suffer from low yields. In particular, cNDs can stabilize large patches of lipid bilayers for the reconstitution of complex membrane biochemical reactions, enabling the capture of crucial intermediates involved in synaptic transmission and viral entry. Circularized nandiscs (cNDs) exhibit superb monodispersity and have the potential to transform functional and structural studies of membrane proteins.
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